Best vectors for gene mapping (FISH)?

AI Thread Summary
When considering the best vector for gene mapping using FISH, the distinctions between plasmids, cosmids, BACs, and YACs are crucial. Plasmids, derived from bacteria, are smaller and suitable for simpler applications. Cosmids, originating from viruses, can carry larger inserts than plasmids but are still limited. BACs (Bacterial Artificial Chromosomes) are larger than cosmids and are effective for cloning larger DNA fragments. YACs (Yeast Artificial Chromosomes) can accommodate the largest inserts, making them ideal for complex gene mapping. The choice of vector depends on the gene of interest, the required insert size, and the expression system, with YACs being favored for their capacity to handle larger genomic regions.
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If you were to have clones of genes you wished to use for FISH in the form of a plasmid, cosmid, BAC and YAC, which would be best for gene mapping? I'm unsure as to what the distinction would be between these types particularly for use in FISH. Which is most commonly used and why?

Thanks
 
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I will try to help.

Plasmids are from bacteria source,cosmid from viruse,YAC from yeast. So it depends from where and which gene are you want to see with FISH? Difference is expression promoters..ect,then there is an issue of scale or number of genes in those systems,YAC is biggest, so bacteria is procaryotic and yeast eucaryotic it is big difference as you probably know.
 

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