Finding mass of glucose in standard solutions

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Discussion Overview

The discussion revolves around a homework problem related to an experiment using thin layer chromatography (TLC) to separate and identify sugars in a sample. Participants are tasked with determining the mass of glucose in standard solutions, given specific concentrations of glucose and other sugars.

Discussion Character

  • Homework-related

Main Points Raised

  • One participant states that they need to convert the concentration of glucose from mg/ml to μg/ml or μg/μl, expressing uncertainty about the wording of the question and its implications for the solution.
  • Another participant highlights the ambiguity in the question, suggesting that without the exact wording, it is difficult to provide a definitive answer.
  • A third participant clarifies that the provided glucose concentration is 1 mg/ml, while the standard solutions contain glucose at 10 mg/ml, indicating a potential misunderstanding about the presence of glucose in the other solutions.
  • A later reply asserts that, based on the previous messages, there is no glucose in the other standard solutions, which raises questions about the interpretation of the problem.

Areas of Agreement / Disagreement

Participants do not reach a consensus, as there are differing interpretations regarding the presence of glucose in the standard solutions and the requirements of the homework question.

Contextual Notes

The discussion lacks clarity on the specific wording of the homework question, which may influence the understanding of the problem. There are also unresolved assumptions about the definitions of reducing sugars and the implications of the given concentrations.

DespicableMe
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Homework Statement



This is for an experiment that separates sugars in a sample using TLC.
We are given a glucose standard solution with a concentration of 1mg/ml as well as 3 standard sugar solutions (glucose, fructose and maltose) all with the same concentration (10mg/ml) in 10% v/v isopropanol)

We are asked to find μg of glucose (reducing sugar) in each of the standards.

Homework Equations


I don't think there are any equations, just conversions.

The Attempt at a Solution


Convert the mg/ml to μg/ml or to μg/μl
But I'm not sure if I'm overthinking it or if it's the way the question is worded because it's worth only 1 mark.
 
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Hard to say anything not knowing how the question is worded. From your description it can be anything and everything.
 
We're given a juice sample and we use thin layer chromatography to identify the sugars in there. They gave us the concentrations of the standard sugar solutions and we were asked to find the μg of glucose (the reducing sugar) in each of the standards. They said to refer to the glucose concentration given to us which was 1 mg/ml. The concentration of the standards were 10 mg/ml though.
 
From what you wrote there is no glucose in the other standard solutions.
 

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