Fluorescent Microscopy: Can Antibodies Be Visualized?

  • Thread starter Thread starter TytoAlba95
  • Start date Start date
  • Tags Tags
    Microscopy
AI Thread Summary
Fluorescent microscopy can indeed be used to visualize fluorescently tagged antibodies, a technique widely employed in biological research. Various methods exist for fluorescent labeling, including biotin, enzymes, and gold particles, which share similar technical principles. This versatility allows researchers to utilize a range of labeling techniques for effective visualization in their studies.
TytoAlba95
Messages
132
Reaction score
19
TL;DR Summary
Can a fluorescent tagged antibody raised against a protein be visualized under fluorescent microscope?
I'm aware that GFP tagged protein can be visualized under fluorescent microscopy. I don't know if it is possible with fluorescent tagged antibodies also. I did some research but couldn't find any information about this.

The answer provided is no.

(pg 25, sum no.142)
 
Biology news on Phys.org
Yes it can.
Fluorescent labeling with antibodies is a commonly used technique in many biology research labs.
Pictures.
There are a huge number of different techniques that can be used.

Similar technical principles will also apply to different labeling methods, such as labeling with biotin, enzymes or gold particles.
 
  • Like
Likes TytoAlba95
Thank you so much.
 

Thread 'Flesh-eating New World Screwworm re-occurrence in US'

Deadly cattle screwworm parasite found in US patient. What to know. https://www.usatoday.com/story/news/health/2025/08/25/new-world-screwworm-human-case/85813010007/ Exclusive: U.S. confirms nation's first travel-associated human screwworm case connected to Central American outbreak https://www.reuters.com/business/environment/us-confirms-nations-first-travel-associated-human-screwworm-case-connected-2025-08-25/...
View full post »

Thread 'Chagas disease now established in US'

Chagas disease, long considered only a threat abroad, is established in California and the Southern U.S. According to articles in the Los Angeles Times, "Chagas disease, long considered only a threat abroad, is established in California and the Southern U.S.", and "Kissing bugs bring deadly disease to California". LA Times requires a subscription. Related article -...
View full post »

Thread 'I need help understanding this quote about recent Natural Selection'

I am reading Nicholas Wade's book A Troublesome Inheritance. Please let's not make this thread a critique about the merits or demerits of the book. This thread is my attempt to understanding the evidence that Natural Selection in the human genome was recent and regional. On Page 103 of A Troublesome Inheritance, Wade writes the following: "The regional nature of selection was first made evident in a genomewide scan undertaken by Jonathan Pritchard, a population geneticist at the...
View full post »

Similar threads

Explain Different Types of Light Microscopy
Replies
4
Views
2K
Fluorescence Microscopy & Antibody Staining
Replies
6
Views
9K
How Do Cryo-EM Images Enhance Our Understanding of the Coronavirus?
Replies
1
Views
1K
The molecular switch from welcoming to attacking a pathogen?
Replies
6
Views
2K
What Unique Microscopy Samples Can Engage Kids in STEM Learning?
Replies
4
Views
2K
Negative amplitude of cross-correlation function in ICCS?
Replies
6
Views
2K
Mathematical process for protein folding
Replies
5
Views
3K
Airy disk and resolution in confocal microscopy
Replies
10
Views
6K
What strategies can a visual learner use to excel in science and mathematics?
Replies
1
Views
504
Detection of a Specific Fluorescent Wavelength via iPhone camera
Replies
3
Views
6K

Hot Threads

Recent Insights

Back
Top