1. Folding can be described as transition between the native and unfolded state. In increasing concentrations of denaturant, a fold disintegrates with a rate lnKU = ln KU H20 - m ku [denat] when the concentration of denaturant is above that needed to unfold 50 % of protein. Conversely folding takes place with rate lnKf = ln Kf H2O - m ku [denat] when the concentration of denaturant is below that needed to unfold 50 % of protein. Write an equation for the observed rate constant ln kobs and plot it (Chevron plot) as a function of denaturant concentration. How do you determine from the figure kfH2O? How will the Chevron plot change when a mutation will affect the transition state?