In the famous equation, E stands for energy, m for mass, and c for the speed of light. "In the test, we at MIT measured m, or rather the change in m associated with the energy released by a nucleus when it captures a neutron," said former MIT graduate student Simon Rainville.
The NIST/ILL scientists, led by Hans B̦rner of ILL and the late Richard Deslattes of NIST, measured E. (The speed of light is a defined and therefore exactly known quantity, so it was simply plugged into the equation.)
Specifically, the ILL/NIST team determined the energy of the particles of light, or gamma rays, emitted by the nucleus when it captures a neutron. They did so using a special spectrometer to detect the small deflection of the gamma rays after they passed through a very pure crystal of silicon.
The mass loss was obtained at MIT by measuring the difference between the mass of the nucleus before the emission of a gamma ray and after. The mass difference was measured by comparing the cyclotron orbit frequencies of two single molecules trapped in a strong magnetic field for several weeks.