Assuming the internal resistance of a cell is zero can lead to significant errors in circuit output, depending on the application. For low current draws, such as powering a small resistor and LED, the voltage drop due to internal resistance is negligible, making the assumption acceptable. However, for high current applications, like powering a 50W light bulb, the internal resistance causes a substantial voltage drop, making the assumption inaccurate. Additionally, internal resistance can vary as the battery discharges, affecting voltage output under load. Therefore, the validity of assuming zero internal resistance hinges on the specific application and required accuracy.