LacZ E coli: Galactoside Permase Activity Without Glucose

Thread starter Persefone
Start date Jun 16, 2005
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The discussion centers on the lacZ gene in E. coli and its failure to exhibit galactoside permase activity when lactose is added in the absence of glucose. It is noted that the expected behavior of the lac operon suggests that both glucose and lactose should inhibit activity, rather than lactose alone. This indicates a misunderstanding of the regulatory mechanisms of the lac operon, which typically requires the presence of lactose to induce expression, but glucose's presence can repress it. The linked resource provides additional insights into the functioning of the lac operon and its regulatory dynamics.

Jun 16, 2005

Persefone

It's about lacZ E coli's failure to showing galactoside permase activity after adding lactose without glucose's presence. why ?

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Jun 17, 2005

detta

http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/L/LacOperon.html

This site will probably help you
i would recheck your question. it seems that the activity should be off with both glucose and lactose.. not with just lactose

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