Hi all I am doing this lab in my organic course right now, and I have a few questions. The phenol I am working on is pKa of p-chlorophenol. I obtained a UV spectrum of phenol in acid, phenol in base, phenol in buffer with higher pH and phenol in buffer with lower pH. 1)In the UV spectrum, there is a isosbestic or crossover point by the 4 solutions used above, would changing of the pH and concentration alter this point ? 2)On my UV spectrum the absorbances of phenol and phenolate is different, is it because one of them are conjugate acid and other is conjugate base? 3)Finally a stupid question, Why is not right to wash the glassware with acetone ? I know it is a very strong and reactive solvent than phenol, but what properties makes it so ? I am sorry if these question are stupid, but my prof didn't went over any of these stuff. Thanks in advance.