Raman Microscopy: Best Way to Hold Micron-sized Polystyrene Beads

  • Thread starter Thread starter n0_3sc
  • Start date Start date
  • Tags Tags
    Microscopy Raman
AI Thread Summary
The discussion focuses on the optimal methods for holding micron-sized polystyrene beads during Raman microscopy. It highlights the use of laser tweezers for optical trapping, which can automatically hold particles in place while still requiring microscope slides for support. References to relevant literature, such as D.V. Petrov's work on Raman spectroscopy and optical traps, emphasize the established techniques in the field. The conversation indicates a need for practical solutions in experimental setups involving these particles. Overall, effective sample handling is crucial for successful Raman microscopy of micron-sized beads.
n0_3sc
Messages
238
Reaction score
1
What's the best way to hold a sample of micron-sized polystyrene beads for raman microscopy?
 
Physics news on Phys.org
With a laser tweezer:

Raman spectroscopy of optically trapped particles
D V Petrov 2007 J. Opt. A: Pure Appl. Opt. 9 S139-S156 doi: 10.1088/1464-4258/9/8/S06
 
Already know about that...seems I'm going to have to use microscope slides...
 
After reading that article and other articles regarding optical traps and microscopy, it seems that the optical trapping is an automatic process? Ie. you still need the cover slides but the particles are automatically trapped by the beam.

"Optical Trapping and Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopy of Submicron-Size Particles"
IEEE JoQE Vol.11 No. 4 Jul/Aug 2005
 

Thread 'The Physics of unloading sand from a barge'

I think it's easist first to watch a short vidio clip I find these videos very relaxing to watch .. I got to thinking is this being done in the most efficient way? The sand has to be suspended in the water to move it to the outlet ... The faster the water , the more turbulance and the sand stays suspended, so it seems to me the rule of thumb is the hose be aimed towards the outlet at all times .. Many times the workers hit the sand directly which will greatly reduce the water...
View full post »

Similar threads

Raman Microscopy - simple question
Replies
4
Views
3K
Measuring the inductance of a micron feature size gold coil
Replies
5
Views
2K
Nanopores for sequencing DNA - question on beads?
Replies
4
Views
3K
Trouble taking Raman spectra in solution.
Replies
2
Views
2K
Statistics of charged beads in electric field
Replies
3
Views
2K
Best way to go for precision aluminum parts?
Replies
18
Views
3K
Rotational Physics - Bead on spinning ring
Replies
5
Views
5K
Theory of Structured illumination microscopy
Replies
3
Views
2K
A Mode Matching a Cavity: Best Practices Explained
Replies
2
Views
3K
Ionising Particles Quickly: What's the Best Way?
Replies
21
Views
2K

Hot Threads

Recent Insights

Back
Top