There is a lepton analogue called the MNS matrix, for Maki, Nakagawa and Sakata.
The CKM (and MNS) matrices are unitary by construction. Essentially they are a change in basis - a rotation, if you will - so must be unitary. A separate question is experimental: is the 3x3 matrix that we can measure unitary? If the answer is "no", that's proof for new physics: e.g. a 4th family.
For quarks, the answer is that we cannot tell. Partly this is because the effect of new physics can be made arbitrarily small, but there's a little more to it than that. For example, given only the upper 2x2 CKM matrix, one cannot infer the existence of a 3rd family. Additionally, the numbers don't work out nicely: Vtb is so close to 1 that one needs a measurement of single top production good to better than 0.1% to see the effect of other quarks.