The harmonic number in standing waves is determined by the largest wavelength that fits within specific boundary conditions, such as open or closed ends. The fundamental frequency, or first harmonic, corresponds to the longest wavelength that can resonate in the given space. For pipes, the harmonic number is influenced by whether the ends are open or closed, with specific wavelengths corresponding to each harmonic. In laboratory settings, a single frequency is typically applied to create a standing wave, while real-world scenarios often involve multiple harmonics occurring simultaneously. Ultimately, the presence of damping and the applied frequency dictate which harmonics are established.