What are reflectance spectra and synchronous spectra?

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Reflectance spectra and synchronous fluorescence spectra are analytical techniques used primarily in the study of plant and animal tissues. Reflectance spectra measure the intensity of light reflected off a material at various wavelengths, providing insights into the concentrations of substances like chromatin and hemoglobin. In contrast, synchronous fluorescence spectra involve a scanning or rastering approach to capture fluorescence data based on positional measurements. This method allows for a more detailed spatial analysis of the fluorescence emitted by the sample. Both techniques are valuable for understanding the composition and characteristics of biological materials.
dmaya
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What are reflectance spectra and synchronous spectra? What are the difference and which information I can obtain from them ?
 
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Welcome to Physics Forums dmaya!

Please be sure to read our guidelines; I notice that you've posted this same in another section - I'll be locking that.
 
I think this thread will fare better in Biology or Chemistry. As far as I'm aware, both reflectance as well as synchronous fluorescence spectra are used most commonly, to study plant and animal tissue.

Reflectance spectra simply record the intensity vs. wavelength data (normalized with respect to the source) for the light reflected off some material. Typically, such spectra tell you things about chromatin or haemoglobin concentrations...but a biologist might be able to answer this better.

I think synchronous spectra involve a scanning/rastering measurement that gives fluorescence data as a function of position. This is just a vague recollection, so I repeat that this thread might be better served if moved to Biology or Chemistry.
 

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