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Biology - Calculating Resolving Power of Microscope?

  1. Sep 15, 2009 #1
    Biology -- Calculating Resolving Power of Microscope??

    1. The problem statement, all variables and given/known data
    The question is this:

    Resolving power is a function of the wavelength of light used and the numerical aperature of the objective lens. Given that RP= wavelength / (2 x NA), calculate the resolving power of the microscope when using oil immersion lens. Visible light has an average wavelength of 0.55 um ( 1 um = 0.000001 meters)

    :/ Help? I think I got it but I'm not sure what to use for NA.


    2. Relevant equations


    RP= wavelength / (2 x NA)

    3. The attempt at a solution

    RP= (0.55 um x 0.000001 meters) / (2 x 1.3 )

    I found that 1.3 value online. I don't even understand how to get that value...
     
  2. jcsd
  3. Sep 15, 2009 #2

    mgb_phys

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    Re: Biology -- Calculating Resolving Power of Microscope??

    The numerical aperture depends on the focal length of the objective (and it's design)
    Were you given the magnification?
     
  4. Sep 18, 2009 #3

    chemisttree

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