Enzyme concentration on the rate of hydrolysis of casein

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The discussion focuses on investigating how varying enzyme concentration affects the rate of hydrolysis of casein. The original poster is seeking mathematical expressions to determine the rate of reaction based on enzyme concentration, noting that most available information emphasizes substrate concentration. A suggestion is made to consider the Michaelis-Menten equation, which describes the rate of enzymatic reactions and incorporates both enzyme concentration and substrate concentration. The equation provided is V = (k2[E]t[S]) / (KM + [S]), which can be useful for analyzing the relationship between enzyme concentration and reaction rate.
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I am currently working on an investigation on the effects of varying enzyme concentration on the rate of hydrolysis of casein. I have looked on the internet to work out the maximum rate, but they all depnd on one variable - substrate concentration. I cannot find any mathematical expressions to work out rate by altering the concneration of enzyme. Can anyone help please??
 
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How about the Michaelis-Menten equation?

V=\frac{k_2[E]_t<s>}{K_M+<s>}</s></s>
 
thank you very much
 

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