(adsbygoogle = window.adsbygoogle || []).push({}); 1. The problem statement, all variables and given/known data

The binding of a ligand B to a protein is studied. The protein concentration it 10^-3 M. The amounts bound,b, for each addedis given as the following:

(M) (b)(M)

.001 5*10^-6

.002 3.3*10^-3

.005 3.78*10^-3

.007 4.72*10^-3

.01 4.95*10^-3

.02 5*10^-3

.05 5*10^-3

Find if it is cooperative, the number of binding sites, and K_d

2. Relevant equations

The hills equation can be expressed as log Y/(1-Y) =n log [A] - log K_d

3. The attempt at a solution

So the y intercept is - log K_d and the slope is n when you plot the left side in respect to log [A]. Y is the fractions filled, so I think it would be (b)/(5*10^-3). But, plotting the graph.. it seems a little screwy.. the plot goes from slope of 10.9 to .5 then to ~5.. The graph is shown here.

1. The problem statement, all variables and given/known data

2. Relevant equations

3. The attempt at a solution

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# Homework Help: Hill's equation and plot-Biochem

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