Negative potential difference in an electric circuit can be achieved by referencing measurements to different terminals of a battery. When the negative terminal is connected to ground potential, voltages measured at various points will appear positive. Conversely, if the positive terminal is used as the reference point, the voltages will read negative. This concept parallels gravitational potential, where moving upwards increases potential and moving downwards decreases it. Understanding this principle is essential for accurately interpreting voltage measurements in circuits.