To apply a non-uniform sinusoidal load on a cylinder in ANSYS, users can utilize APDL (ANSYS Parametric Design Language) to define the load based on node or element coordinates. APDL allows for batch scripting, enabling the definition of custom load functions such as sin(x). Detailed documentation is available to assist in understanding the implementation process. Users can also request sample APDL files for practical guidance. The discussion emphasizes the need for applying consistent loads across coordinates on the cylinder.