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sotellme
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does anyone know what an ORF (open reading frame) is?
it would help me a lot if anyone explains this to me.
thank you so much!
it would help me a lot if anyone explains this to me.
thank you so much!
An open reading frame (ORF) is a portion of a gene's sequence that contains a sequence of bases, uninterrupted by stop sequences, that could potentially encode a protein.
iansmith said:In eukaryotes, ORF are usually th...op codon? hope for reply! thanks again!
iansmith said:an exon has a start and a stop sequence to mark the exon
An open reading frame (ORF) is a portion of a gene’s sequence that contains a sequence of bases, uninterrupted by stop sequences, that could potentially encode a protein. When a new gene is identified and its DNA sequence deciphered, it is still unclear what its corresponding protein sequence is. This is because, in the absence of any other knowledge, the DNA sequence can be translated or read in six possible reading frames (three for each strand, corresponding to three different start positions for the first codon). ORF identification involves scanning each of the six reading frames and determining which one(s) contains a stretch of DNA sequence bounded by a start and stop codon, yet containing no start or stop codons within it; a sequence meeting these conditions could correspond to the actual single product of the gene. The identification of an ORF provides the first evidence that a new sequence of DNA is part or all of a gene encoding for a particular protein. Source : Celera
An ORF (Open Reading Frame) is a DNA sequence that can be translated into a protein. It is typically defined as a sequence of nucleotides that starts with a start codon (ATG) and ends with a stop codon (TAA, TAG, or TGA). These sequences are important for identifying potential protein-coding genes in a genome.
An ORF is a sequence of DNA that has the potential to code for a protein. It is different from a gene, which is a functional unit of DNA that includes regulatory elements, introns, and exons. Genes can contain multiple ORFs, and not all ORFs are necessarily genes.
Yes, an ORF can be found in non-coding regions of DNA. While the majority of ORFs are found in protein-coding genes, there are also ORFs in non-coding regions that may have other functions, such as regulatory roles.
ORFs are typically identified using bioinformatics tools that search for sequences that meet the criteria for an ORF, such as starting with a start codon and ending with a stop codon. These tools can also predict the size and potential function of the ORF based on the nucleotide sequence.
ORFs are important in genetics and genomics research as they can help identify potential protein-coding genes and provide insights into the function and evolution of a genome. They are also useful in gene annotation and can aid in the discovery of new genes and their functions.