The discussion centers on introducing variable pressure into a system using piston pumps, with the goal of achieving controlled pressure fluctuations for an experiment. The user has attempted to create pressure pulses using a stepper motor and cam arrangement but is struggling to achieve higher frequencies. They have flow rates between 0.01 to 0.09 ml/min and are currently using a syringe pump. Suggestions include providing more details about the system's requirements and considering the use of a fast-acting valve to generate pressure pulses instead of relying solely on a piston pump. The conversation emphasizes the need for clarity on pressures, fluid type, and desired pulsation frequency.