- #1
Steve Drake
- 53
- 1
Hi Guys,
I am working on some fluorescence correlation spectroscopy and I have some data generated by the machine I use. The data has the correlation plot of G(tau) vs the delay time (tau) which is an exponential decay.
The data also has the count rate given for the duration for the experiment (240 readings over a 120 second experiment).
Ive read about the auto correlation function and would like to generate it by hand (using MATLAB or excel or whatever) to get the same graph. The problem I have is all of the formulas I have been using don't seem to give a nice graph that starts nicely correlated, then decays down to 0 (like this: http://www.fcsxpert.com/classroom/theory/whatisfcs_pics/image038.gif)
(The formulas are like the one on this site http://www.fcsxpert.com/classroom/theory/what-is-autocorrelation.html)
Could anyone please explain how i would set up a program to generate that decay curve from the intensity fluctuations using the auto correlation function?
Thanks
I am working on some fluorescence correlation spectroscopy and I have some data generated by the machine I use. The data has the correlation plot of G(tau) vs the delay time (tau) which is an exponential decay.
The data also has the count rate given for the duration for the experiment (240 readings over a 120 second experiment).
Ive read about the auto correlation function and would like to generate it by hand (using MATLAB or excel or whatever) to get the same graph. The problem I have is all of the formulas I have been using don't seem to give a nice graph that starts nicely correlated, then decays down to 0 (like this: http://www.fcsxpert.com/classroom/theory/whatisfcs_pics/image038.gif)
(The formulas are like the one on this site http://www.fcsxpert.com/classroom/theory/what-is-autocorrelation.html)
Could anyone please explain how i would set up a program to generate that decay curve from the intensity fluctuations using the auto correlation function?
Thanks