To activate a relay using a phototransistor, it's important to note that the phototransistor may not provide sufficient current due to its high resistance, even in bright light. An amplifier circuit is recommended to boost the current, and using a small power transistor like a TIP31 or BD139 can be effective. Adjusting the voltage to around 9 volts and including a series resistor of about 1000 ohms may improve performance. For reversing the relay action, specific resistor values must be calculated to ensure the transistor receives adequate base current. Properly configuring the circuit will allow the relay to operate based on the phototransistor's state in varying light conditions.