To create a 25 mM Tris-phosphate buffer at pH 7.8, one can combine 25 mM Tris-base with 25 mM phosphoric acid. The pH can be adjusted to the desired level using phosphoric acid. There is some confusion regarding whether Tris-base and phosphoric acid are conjugate pairs, as they are not, but they can still function together in a buffering system. The discussion highlights the use of Tris-phosphate buffers in applications like gel electrophoresis, raising questions about the interaction between Tris and phosphoric acid and how their concentrations are maintained in the final buffer solution. It is noted that if the two components do not react, the concentrations of each would remain unchanged, resulting in a solution that retains the original concentrations of both Tris and phosphoric acid.
