The discussion revolves around calculating the potential difference in a varying electric field using the integral of the electric field. The user initially questions whether the initial potential can be assumed as zero and seeks clarification on limits of integration and the path from point A to B. It is emphasized that the path chosen affects the calculation, with a suggestion to break it down into segments for easier evaluation. The correct differential along the x-axis is identified as dx, and the user is guided through setting up the integral properly. Ultimately, the sign of the potential difference is clarified by discussing the direction of electric field lines in relation to potential.