Hi, I have a quick question on fluorescence. I have fluorescein molecule. Its absorbance is pH sensitive. However, I want to know if 1. It emits fluorescence. 2. the fluorescence intensity pH sensitive I know for sure it emits fluorescence. I see, what I believe to be an emission peak. However, I am not able to get any pH response. Irrespective of how acidic or basic I get, I am unable to get the intensity to change. Can anyone explain this. Can we say by default that if a molecule has fluorescein in its structure, it should fluoresce? And, if it fluoresces, then it should be pH sensitive too, given, the absorbance is pH sensitive. Thanks for your help.