To calculate the maximum deflection of a bike axle, the formula (W*a/24*E*I)*(3*l^2-4*a^2) is proposed, with the effective span being the clear distance between the wheel flanges, which is 370mm. The discussion clarifies that the total length of the axle does not need to be included in this calculation. The axle is confirmed to be part of a tricycle, with collars acting as bearing housings to support the load. The inertia of the frame is calculated as 19000mm^4, using the formula for a solid cylindrical shaft, and the load is approximately 700N with E for aluminum at 70GPa. The conversation highlights the importance of accurate measurements and the correct application of the formula for deflection.