Rujano said:I now that I have to substract that value to to each of the other absorbances that I have
A calibration curve (absorbance) is a graphical representation of the relationship between the concentration of a substance and its corresponding absorbance value. It is used to determine the concentration of an unknown sample by comparing its absorbance to the curve.
A calibration curve is important because it allows for accurate and precise determination of the concentration of a substance in a sample. It takes into account any variability in the instrument and helps to correct for any errors in the measurement process.
A calibration curve is created by measuring the absorbance of a series of known concentrations of a substance and plotting them on a graph. The resulting curve is then used to determine the concentration of an unknown sample.
Yes, a calibration curve can be used for multiple substances as long as they have a similar absorbance behavior. However, it is recommended to create a separate calibration curve for each substance to ensure accuracy.
The accuracy of a calibration curve can be ensured by using a sufficient number of data points, properly labeling and identifying each point, and using high-quality standard solutions. It is also important to regularly calibrate and validate the instrument used to create the curve.