Which method would be wrong to use for gene expression?

  • #1
2
0
Homework Statement:
Which of the following techniques is not appropriate if you want to examine whether a gene is expressed in a
specific tissue?

Select one alternative:
in situ hybridization
immunohistochemistry
microarray
RNA-Seq
Relevant Equations:
Non
I've been struggling, no matter what I try to do I cant come up with a solution. The answer is in situ, but in my mind it would work perfectly fine. The only thing we would do is just use an RNA probe complementary to the gene we are trying to detect and it should light up the moment the gene is expressed.
 

Answers and Replies

  • #2
Ygggdrasil
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I agree that in situ hybridization to detect the mRNA transcripts from the gene of interest would work. The one reason I could see why they might say that ISH is the wrong technique to use is if they are referring to DNA detection by ISH (but because ISH can be used to detect either DNA or RNA, the question is not well stated if that is the reasoning they intended).
 
  • #3
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But if we use that reasoning would immunohistochemistry also be wrong since it detect protein production and not actual gene expression. A gene can still be expressed but does not have to produce protein.
 

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