Microscope Definition and 252 Threads

Why is the focal length of the objective lense is less than that of the eye piece in a compound microscope?

thankyou to anyone reading this. I am starting honours in physics and have been given the opportunity to do a project that leads to a phd in the condensed matter theory. The guy I will be working with seems to specifically do work around the theory involved in electron microscopes. hes a great...

I am using a Renishaw inVia confocal microscope to study emission spectrum of molecules. The excitation wavelength of laser that I use is 532nm. However, I met with a very basic problem. When I use the same material (say plain silicon), and use different objectives (20X, 50X, 100X), then I will...

Homework Statement Basically, derive the formula ## m = \frac{ 25 cm}{f_e} \frac{L}{f_o} ## using ray matrices. This just has variable tube length and assumes eye to object distance is 25 cm. Homework Equations Ray matrices: ## \left[ \begin{array}{cc} 1 & d \\ 0 & 1 \end{array} \right] ##...

Can the Heisenberg's Gamma Ray Microscope thought experiment derive the Uncertainty Principle precisely? Most derivations I find, the uncertainty is roughly 2h, whereas the uncertainty principle is "hbar over 2". Is there anywhere where there is more precise calculations to obtain "hbar over 2"...

I have isolated a large molecular complex whose integrity is very sensitive to increasing ionic strength and thus had to be prepared for EM using low ionic strength solutions. And my goal is image this by electron tomography by collecting a dual tilt series of 120 images. Other experimental...

I have recorded a micrograph of a 2-D array at a magnification of 43,000x on my DE-20 digital camera, which has a 6.4 μm pixel size and a frame size of 5120 × 3840 pixels. This magnification is correct at the position of the camera. I then compute the Fourier transform of the image. What is the...

Homework Statement The barrel of a compound microscope is 15.9 cm in length. The specimen will be mounted 1.17 cm from the objective, and the eyepiece has a 5.00 cm focal length. Determine the focal length of the objective lens.Homework Equations I am using the mirror equation. The Attempt at...

Homework Statement First, thanks in advace. Let us consider a microscope where the objective L1 has f1=20mm and magnification 10x. In the image plane is located a diafragm M with diameter 19mm (see fig). The size of the CCD is 4,8mm (vertical) x 5,6mm (horizontal). 20mm before of the CCD...

Is there any usage for a measuring microscope in an electricity laboratory as a part of any experiment? We have a Mitutoyo measuring microscope in our fundamental electricity physics lab which is a course for sophomores. We used it for apparent depth in our optics lab which is retired now! I'm...

is magnification negative for a compound microscope at infinity

Not sure if this is the right place for this thread but, have you guys ever made anything cool at home? I was planning on making an inverted microscope out of a webcam by just taking the lens out and flipping it over and setting it up like a microscope.

I noticed that the ray diagram for the "how the compound microscope works" and "how the telescope works" is wrong in my government sanctioned physics textbook(and countless other books and websites!) The diagram printed was this- Name: Physics Standard XI Printed by: Maharastra State Board of...

Homework Statement Your molecular biology lab studies proteins, and you're frustrated because your microscopes can't quite resolve crystallized proteins. A sales rep touts the advantages of an expensive microscope using 200-nm ultraviolet light, saying you'll be able to resolve structures less...

I can only find micrographs (photographs of microstructure under electron microscope) of non-transparent glasses. Is it impossible top be able to see the internal structure of a transparent material? If anyone can tell me what I'm doing wrong or find me one that would be great! I'm looking...

Homework Statement The problem along with its solution is attached as ProblemSolution.jpg. Homework Equations Δx = λ/sinθ (Eq. 1) Δp_x = (h/λ)(sinε) (Eq. 2) The Attempt at a Solution In Wikipedia, I found this article: http://en.wikipedia.org/wiki/Heisenberg's_microscope which uses...

Hi there As is known using non-infinity corrected objectives in infinity corrected microscopes is not a good idea because of many reasons (image quality will be degraded, back focal plane will be in wrong place, parfocal distance is not preserved, etc.). What about building infinity corrected...

Homework Statement You are building a compound microscope with an objective lens of focal length 0.70 cm and an eyepiece lens of focal length 5.0 cm. You mount the lenses 18 cm apart. What is the maximum magnification of your microscope? Homework Equations...

What does the piezoelectric scanner do? Does it record the electric current tunnelled by the tip or is it just used to move the tip in response to electric currents?

Dear All I am trying to measure the spectrum of an incandesant 6V 15W tungsten microscope lamp. I have a reasonably old scanning monochromator (diffraction grating based) coupled to a fibre optic cable and it has given me the spectrum...

Is it possible to see twins in Si under an optical microscope?

I'm not asking for what reflection and refraction are or the usual law governing it, but I would like to understand what they represent at the quantum atomic, molecular level? In a mirror is it about photons absorbed and emitted with the same wavelength and same direction through atomic electron...

Homework Statement A microscope has a 13.0 x eyepiece and a 57.0 x objective lens 20.0 cm apart. Calculate the focal length of each lens. Where the object must be for a normal relaxed eye to see it in focus? Homework Equations M=m(objective)*m(eyepiece)=f(objective)/f(eyepiece)...

what is the depth of field of a microscope and how is it different from the depth of focus of the microscope.

Why in telescopes and microscopes the ocular distance is such that it creates virtual images instead of real images?

1. Homework Statement A Homemade compound microscope has, as objective and eyepiece, thin lenses of focal lengths 1cm and 3cm, respectively. An object is situated at a distance of 1.20cm from the objective. If the virtual image produced by the eyepiece is 25cm from the eye, compute (a.) the...

Hi Guys, Ive been doing some work with E. Coli recently and I would like to image them under a scanning electron microscope. There are lots of papers with lots of different methods to prepare them for such a feat... but has anyone here actually done it and gotten a decent image? If so what...

Hi, I am looking for some detailed electron microscop images of HIV virus itself. I need it bigger and more detailed then this: http://www.allvoices.com/contributed-news/9114517/image/80168820-hiv-virus-electron-micro-graph I would like to have some picture, not computer render or...

Looking for a rough distance scale over which statistical methods begin to work, and macroscopic thermodynamic variables begin to make sense. Thanks

My text explains that free float frames are locally indistinguishable from flat space-time in the sense that in a small enough region measuring instruments will be unable to detect gravitational tidal forces. This region may be tiny if tidal forces are large or if measuring instruments are...

I have been using a relatively cheap microscope (My first Lab Microscope) to look at a sample of my semen. Under 40x magnification I see a lot of motionless sperm and generally not enough sperm floating about compared to what I see from other microscope videos of sperm floating (no pun) on the...

Homework Statement To examine a particular blood sample in biology lab, a student uses a compound microscope set to have a magnification of -4530. The objective and eyepiece are both attached to a tube which is 18 cm in length and the eyepiece has a focal length of 2.62 cm. The near point of...

I know an electron microscope can collect x-rays emitted by electron holes, backscattered electrons as well as secondary electrons. I get that an electron's wavelength is much smaller than a photons, thus you get finer image resolution, but how exactly does a shorter wavelength mean a finer...

I just link my microscope (Olympus CX31) with CCD (Sony), the staff 's company installed "Ulead program" in computer linked with CCD to capture the picture from microcope. The problem is when the picture shown in the computer, it is already too enlarged that we can't see the wider view as we...

For oil immersion microscopy, I understand that the oil is required to increase the numerical aperture, and thus the resolution, of the lens. I have tried looking into these microscopes and the only method I have seen of applying the oil is to place a dot of it on the lens and then lower the...

Homework Statement A microscope has an eyepiece that gives an angular magnification of 5.25 cm for a final image at infinity and an objective lens of focal length 1.45 cm. The tube length is 16.1 cm. a - What is the transverse magnification due to the objective lens alone? b - what is the...

Hi there, Quick question - I know the processes in the microscopic realm are reversible. However, in 'Quantum physics: illusion or reality', Rae says that this is true except for one or two subatomic particle processes. Which particles are these, and why aren't they reversible?

according to wave particle duality particles such as electrons exhibit wave like properties. and the wavelngth is given by lambda=h/p, so increase momentum and get shorter lambda. This property is utilised in the electron microscope to get very short wavelengths and so have very high...

Hello, I am working on researching new ways to insulate some scanning coils on the SEM I work on. Currently we use Kapton to insulate the wires but it becomes brittle and wears over time easily. I am looking for alternatives to using Kapton for the scanning coils. Any ideas and suggestions...

I was wondering if there was a way to build something to see the surface of the moon easily like our satellites can zoom in on us... Is it possible to take a mirror and put it under a microscope that has say 10,000x magnification. Then take a second mirror and reflect the moon onto the mirror...

I don't know where to put this one. The resolving power of a microscope(simple) is defined on the basis of diffraction effects.I have read that and as far as my understanding goes it is 2μsinβ/(1.22λ). This was assuming circular aperture for the lens. [Here 2β is the angle subtended by the...

Hello I have just read first five chapters from Feynman's "Lectures on Physics Vol. 2" on electromagnetism and couldn't find satisfactory answer to my question, so I am posting this question. Its claimed that electric field inside a "perfect conductor" (something with unlimited...

Quantum Physics: Electron Microscope Homework Statement Consider electrons in the accelerating voltage of 15 kV used in an electron microscope with a 'lens' diameter D = 0.5 cm and focal length f= 50 cm. a) We require that the resolution is a tenth of the size of the object we want to...

Homework Statement I've been looking over the internet about Robert Hooke but there seems to be conflicting information about his work. Now I know that he was the first to discover the cell using a microscope and Anton van Leeuwenhoek was the first to discover microbes using a much stronger...

I've read a lot of online articles about buying a microscope, but I guess I'm just looking for some advice. I plan on buying a compound microscope sometime in the near future for hobbyist use. It would be sheerly for my own entertainment. I've been looking at used microscopes on ebay, and...

Hello there folks. So I'm doing some homework, and one of the questions asks for a comparison of the microscopic absorption cross sections of B, Cd, Gd, and Hf with the fission cross section of U-235. I've found the fission cross section in my trusty 17th edition of Nuclides and Isotopes, but...

I just noticed that it's often taken for granted that a macroscopic crystal has a similar geometric structure as in the atomic scale. What is the physical explanation for this (assuming it is true)?

Do molecules, atoms, and subatomic particles have color? If so, what? Is it even possible to determine if they do or not (Can electron microscopes see electrons?)? Is color even relevant at lengths this small? I would elaborate more but these questions are fairly straightforward.

I have recently been learning about the microscope properties which dictates electrical resistance. The main equation (resistivity) in my textbook is: \rho = 2m/qN\tau where \tau is the time between collisions of electrons with the atoms, q is the charge of the electron, N is the number of...

Hi all, could you suggest me references that can help me in the following calculation? I would like to see how to describe microscopically the interaction of light (or in general of an external field) with a disordered system such as glass ( I can find literature only for materials modeled...