Mossbauer data analysis - folding

In summary, the conversation revolves around the folding procedure for raw data from mossbauer spectroscopy. The individual is seeking advice on how to determine the correct sense or direction of folding, as their current software is not producing accurate results. Suggestions are given for using free software or writing a program to fold the data manually, and steps are outlined for identifying the correct folding point. The individual expresses their gratitude for the advice and signs off with their name.
  • #1
NidhiA
1
0
Hi there, this looks like a great forum :-)
I would need help with the folding procedure for raw data from mossbauer spectroscopy. Our software doesn't, for some reason, fold the data correctly, so we want to attempt a manual procedure. But how do we determine the correct sense/direction of folding?
Thanks a lot for advice,
Nidhi
 
  • #3
NidhiA said:
Hi there, this looks like a great forum :)
I would need help with the folding procedure for raw data from mossbauer spectroscopy. Our software doesn't, for some reason, fold the data correctly, so we want to attempt a manual procedure. But how do we determine the correct sense/direction of folding?
Thanks a lot for advice,
Nidhi
Hi,
There are lot of free software for folding (not convolution!) of the the raw data. What software do you use for analysis? But if you want to fold manually, then you should write your own C or FORTRAN program.. For this you should know the theory of folding.
Usually the folding point will be around the central channel.
After geting the raw spectrum choose the centre point and add then find the square of the difference of the counts before and after the centre channel count (symetrically around the selected folding point). Sum the differences and say it as 'x1'. Now take the next point of the central point and repeat the same procedure, and say it as 'x2'..Like wise find 3 point before and after the central point and repeat the procedure..Now see which 'x' is small, then that will be the correct folding point. After finding the folding point add the counts before and after the folding point to get the folded spectrum.
Hope it helps.
Rajini.
 

1. What is Mossbauer data analysis and how is it used in folding studies?

Mossbauer data analysis is a technique used in studying the folding of proteins and other macromolecules. It involves the use of Mossbauer spectroscopy, a type of spectroscopy that uses gamma rays to study the energy levels and electron configurations of atoms. In folding studies, Mossbauer data analysis is used to analyze changes in the electronic structures of atoms in proteins as they undergo folding or unfolding.

2. How does Mossbauer data analysis differ from other spectroscopic techniques?

Mossbauer data analysis differs from other spectroscopic techniques in that it can provide information about the local environment of specific atoms in a molecule. Most spectroscopic techniques only provide an average picture of the entire molecule, but Mossbauer data analysis can distinguish between different electronic environments within the molecule.

3. What is the main advantage of using Mossbauer data analysis in folding studies?

The main advantage of using Mossbauer data analysis in folding studies is its high sensitivity to changes in electronic structure. This allows for the detection of subtle changes in the electronic environment of atoms in proteins as they undergo folding or unfolding, providing valuable insights into the folding process.

4. What are some common applications of Mossbauer data analysis in folding studies?

Mossbauer data analysis has been used in a variety of folding studies, including investigating the folding of proteins, DNA, and RNA, as well as studying the folding of synthetic polymers and surfactants. It has also been used to study the effects of temperature, pressure, and pH on the folding process.

5. What are some limitations of Mossbauer data analysis in folding studies?

One limitation of Mossbauer data analysis in folding studies is that it is limited to studying changes in electronic structure and cannot provide information about the spatial arrangement of atoms in a molecule. It also requires specialized equipment and expertise, making it less accessible compared to other spectroscopic techniques. Additionally, it can only be used to study molecules that contain certain elements, such as iron, in their structure.

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