Nuclear binding energy is directly proportional to the mass defect, which is the difference between the total mass of individual nucleons and the mass of the assembled nucleus. The binding energy represents the energy released when nucleons combine, resulting in a mass decrease. When comparing a nucleus of mass M+1 to one of mass M, the total mass before the combination exceeds the mass after, illustrating the mass defect. This energy difference is often emitted as gamma radiation. Understanding this relationship is crucial for grasping nuclear stability and energy release in nuclear reactions.